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Though H&E stains are relatively simple to perform, there are a variety of problems that can occur. Learn how to troubleshoot common H&E staining issues.
- H&E Staining Overview: A Guide to Best Practices - Leica Biosystems
For routine diagnosis, the use of H&E staining is by far...
- H&E Staining Overview: A Guide to Best Practices - Leica Biosystems
For routine diagnosis, the use of H&E staining is by far preferred for viewing cellular & tissue structure detail. Learn about best practices, protocol & more.
H&E Troubleshooting Table . Page 1 of 7 . Problem Cause Solution . 1. Nuclei not crisp, “smudgy” nuclei, nuclear bubbling or no distinct chromatin pattern seen. There is no variation in the nuclear chromatin patterns among cells, and there is no variation in chromatin staining within one nucleus. a) Fixation is incomplete.
Review the basics of H&E staining and what optimal staining should look like. Identify common problems encountered with staining techniques. Consider solutions to common artifacts that influence stain results.
The ST Infinity H&E Staining System was developed and optimized for use with the Leica ST5010 and ST5020 stainers. Storage and Stability Store reagents at room temperature (15-30°C).
15 maj 2020 · There are numerous variables that can affect the quality of your H&E stain. In this post we will cover some common problems you may run into. The National Society for Histotechnology publishes its blog twice weekly featuring histotechnology-focused topics.
6 wrz 2023 · By utilizing the Guide to Better IHC, you can reference troubleshooting tips for common immunohistochemistry (IHC) staining issues once your staining has been optimized and validated. Learn how to identify staining artifacts such as air-drying and crushed cell artifact, as well as artifacts such as tissue pigments which can often interfere with ...
