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Working distance is the distance between the front of the microscope objective lens and the surface of the specimen or slide coverslip at the point where the specimen is completely in focus. As a general rule working distance decreases and total magnification increases due to the higher numerical apertures associated with high power objectives.
13 mar 2024 · Working distance in a microscope is the required distance between the top of the object and the objective lens that gets your sample in focus. The working distance can vary depending on your lenses and their manufacturers.
13 kwi 2024 · Working distance, a crucial concept in the field of optics and microscopy, refers to the empty space between the object you’re looking at and the front surface of the lens or lenses in a microscope.
Microscope objectives are generally designed with a short free working distance, which is defined as the distance from the front lens element of the objective to the closest surface of the coverslip when the specimen is in sharp focus.
Sample height, or working distance (WD), refers to the distance between the bottom of the SEM column and the top of the sample. Within the sample chamber the sample stage can be wound up closer to the end of the column (a short working distance) or dropped down lower (a long working distance).
Microscope objectives are generally designed with a short free working distance, which is defined as the distance from the front lens element of the objective to the closest surface of the coverslip when the specimen is in sharp focus.
Working Distance - This is the distance between the objective front lens and the top of the cover glass when the specimen is in focus. In most instances, the working distance of an objective decreases as magnification increases.