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Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate , sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate .
Composition and preparation of Phosphate Buffered Saline (PBS) is explained in step-by-step. PBS is used as base component for many other buffers like PBS-T, 4 % PFA etc..
28 sty 2019 · PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical, or biochemical research.
PBS (Phosphate Buffered Saline) (1X, pH 7.4) preparation guide and recipe. Recipe can be automatically scaled by entering desired final volume. PBS is an isotonic buffer frequently used in biological applications, such as washing cells, transportation of tissues, and dilutions.
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells. It is used in several applications of tissue culture, most notably for cell washing and trypsinization.
Alternatively, a 0.1 M phosphate buffered saline (PBS) can be homemade by preparing a solution composed of 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 and 1.8 mM KH 2 PO 4 in deionized water that must be autoclaved before use and can be kept at room temperature.
Overview: Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS supports cell viability by keeping the medium pH and osmolality near-physiological. Several formulations of PBS have been described.
