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Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH.
PBS is an isotonic buffer frequently used in biological applications, such as washing cells, transportation of tissues, and dilutions. PBS closely mimics the pH, osmolarity, and ion concentrations of the human body.
There are many different formulations for phosphate-buffered saline. Some of them contain calcium and magnesium salts. In this page, we are restricting ourselves to provide the most used PBS composition based on (CSHL protocols and Molecular cloning by Sambrook).
28 sty 2019 · PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical, or biochemical research.
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells. It is used in several applications of tissue culture, most notably for cell washing and trypsinization.
PBS is a balanced saline solution that will be used to wash cells to maintain osmotic balance between internal and external environment of cell. From: Methods in Cell Biology, 2023
Phosphate-buffered saline (PBS) PBS can be made as a 1× solution or as a 10× stock. To prepare 1 L of either 1× or 10× PBS, dissolve the reagents listed above in 800 mL of H 2 O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H 2 O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at ...
