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Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate , sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate .
PBS is a balanced saline solution that will be used to wash cells to maintain osmotic balance between internal and external environment of cell. From: Methods in Cell Biology, 2023
PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical, or biochemical research.
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells. It is used in several applications of tissue culture, most notably for cell washing and trypsinization.
Phosphate Buffered Saline (PBS) is a solution prepared by diluting a stock solution of specific salts (NaCl, KCl, Na2HPO4•H2O, KH2PO4) to create a buffer with a pH of 7.3. Tween 20 is added to PBS in concentrations between 0.1% and 0.2% to optimize its performance in biological applications.
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Prepare the following reagents: phosphate-buffered saline (PBS): into 700 ml of distilled water, dissolve 8 g NaCl, 0.2 g KCl, 1.1 g dibasic sodium phosphate, and 0.2 g monobasic potassium phosphate, and then bring to a 1 liter final volume with distilled water; standard fixative: 4% (w/v) paraformaldehyde in 0.1 M phosphate buffer containing 1 ...
