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Phosphate Buffered Saline. In subject area: Biochemistry, Genetics and Molecular Biology. PBS is a balanced saline solution that will be used to wash cells to maintain osmotic balance between internal and external environment of cell. From: Methods in Cell Biology, 2023. About this page.
Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH.
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells. It is used in several applications of tissue culture, most notably for cell washing and trypsinization.
Phosphate buffered saline (PBS) is a commonly used solution in biological research and contains NaCl, KCl, KH 2 PO 4 and Na 2 HPO 4 salts. It has been used as a corrosive medium for testing the applicability of Mg alloys for biomedical application [61, 85–88].
PBS is at the correct osmolarity to keep the cells in an isotonic state. PBS is also often used as a buffer in biochemistry experiments to maintain the pH of proteins. It can be used to dissolve peptide or protein samples directly, and to store the protein or peptide in that solution.
28 sty 2019 · PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical, or biochemical research.
8 lip 2019 · Answer. PBS and dPBS are the abbreviations of phosphate-buffered saline and Dulbecco’s phosphate-buffered saline, respectively. They are well-known buffer solutions that are commonly employed in biological research to maintain a consistent pH (between 7.2-7.6).
