Search results
Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate , sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate .
PBS 1.2 Quiz. Flashcards; Learn; Test; Match; Q-Chat; Flashcards; Learn; Test; Match; ... separate DNA from solution so it regains its form and becomes visible. Restriction Enzymes. cut DNA at specific sequences called recognition sites. Gel Electrophoresis. 1. make gel 2. add DNA 3. "run the gel" (add electricity) 4. visualize DNA (UV light or ...
Chargaff's Rule. the DNA of every organism should display a 1:1 ratio of a certain nucleotide base residue (# thymine=# adenine, #guanine=#cytosine) What are Watson and Crick known for? they determined the shape and structure of DNA (published a paper explaining the three dimensional structure of DNA as a double helix)
28 sty 2019 · PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical, or biochemical research.
The application of the principles of the natural sciences, especially biology and physiology, to clinical medicine. Study with Quizlet and memorize flashcards containing terms like Adenine, Chromosome, Cytosine and more.
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells. It is used in several applications of tissue culture, most notably for cell washing and trypsinization.
There are many different formulations for phosphate-buffered saline. Some of them contain calcium and magnesium salts. In this page, we are restricting ourselves to provide the most used PBS composition based on (CSHL protocols and Molecular cloning by Sambrook).
