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Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate , sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate .
PBS is a balanced saline solution that will be used to wash cells to maintain osmotic balance between internal and external environment of cell. From: Methods in Cell Biology, 2023
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells. It is used in several applications of tissue culture, most notably for cell washing and trypsinization.
PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical, or biochemical research.
Introduction. Phosphate buffered saline (a.k.a PBS or PBS buffer) is one of the most used buffers since it is isotonic to most of the cells. We have placed reagents required for preparation in a table. This table also contains information on molarity of each reagent and amount of salt (s) to be added for making 500mL and 1L of 1X and 10X.
PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation.
Phosphate buffered saline (PBS) is a commonly used solution in biological research and contains NaCl, KCl, KH 2 PO 4 and Na 2 HPO 4 salts. It has been used as a corrosive medium for testing the applicability of Mg alloys for biomedical application [ 61 , 85–88 ].
