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Ligation is the joining of two nucleotides, or two nucleic acid fragments, into a single polymeric chain through the action of an enzyme known as a ligase.
Once the DNA to be cloned exists as a defined fragment (called the target or insert), it can be joined to the vector by the process called ligation. The tool used for this process is DNA ligase, an enzyme that catalyzes the formation of a phosphodiester bond between juxtaposed 5′-phosphate and 3′-hydroxyl termini in duplex DNA.
Definition. Ligation is the process of joining two strands of nucleic acids together, typically through the formation of phosphodiester bonds. This crucial step occurs during RNA splicing, where introns are removed and exons are joined to create a mature mRNA molecule.
What is DNA Ligation? DNA ligation is the process of joining two strands or fragments of DNA molecules through a phosphodiester bond formed by the enzyme DNA ligase. DNA ligase is involved in maintaining the structural integrity of genomes by participating in DNA replication or repair processes.
In the cell, the function of DNA ligase is to covalently link the fragments of the lagging strand during DNA replication, or to repair single-stranded breaks (“discontinuities”) that arise in double-stranded DNA molecules during DNA replication or during DNA damage repair.
Ligation is the process of joining two DNA fragments together through the formation of a phosphodiester bond. This technique is fundamental in genetic engineering and molecular cloning, as it allows the insertion of a DNA fragment into a vector, which can then be introduced into host cells for replication and expression.
Ligation is the process of joining two ends of DNA molecules together, typically using an enzyme called DNA ligase. This process is essential for various cellular functions, including DNA replication and repair, as well as during the creation of recombinant DNA in laboratory settings.
