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Ultraviolet visible (UV-Vis) spectrophotometers use a light source to illuminate a sample with light across the UV to the visible wavelength range (typically 190 to 900 nm).
30 wrz 2015 · In this application-oriented booklet the fundamentals of UV/VIS spectrophotometry are first explained before focussing on its main use in analytical chemistry, and in particular on the ...
In the electromagnetic spectrum shown in the table in the previous page, light with a wavelength of 200-400 nm is called ultraviolet (UV) light, that with 400-800 nm as visible (VIS) light, and that with 800 nm - approximately 1 mm as infrared (IR) light.
A UV-vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.
UV/Vis spectroscopy is a measurement technique in which the recording of the absorption spectra of different samples using ultraviolet (UV) and visible (Vis) light is achieved by a spectrophotometer, i.e. an instrument able to measure the spectrum of a sample in the UV/Vis range.
The basic components for UV/Visible spectrophotometry include a light source, a wavelength selector, a sample compartment (often a cuvette or flow cell) and a detector.
purposes, we choose to characterize light in the ultraviolet and visible regions in terms of wavelength expressed in nanometers. Other units which may be encountered, but whose use is now discouraged, are the Angstrom (Å) and the millimicron (m µ ).
