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The TRAPEZE® Gel-Based Telomerase Detection Kit provides substantial improvements to the original TRAP assay, such as a modified reverse primer sequence which (1) eliminates the need for a wax barrier hot start, (2) reduces amplification artifacts and (3) permits better estimation of telomerase processivity.
The TRAPEZE® XL Kit is a one buffer, two enzyme system utilizing polymerase chain reaction (PCR) and Amplifluor® primers (34). In the first step of the reaction (Figure 1, line A), the telomerase enzyme adds a number of telomeric repeats (GGTTAG) onto the 3' end of a substrate oligonucleotide (TS).
Telomeric repeat amplification protocol (TRAP)—a sensitive, PCR-based assay to detect telomerase activity was quintessential to the evaluation of telomerase role in telomere maintenance, cell proliferation, tumour development, and cell immortalization.
Flow chart of the standard TRAP assay using a single tube reaction. The development of the TRAP assay provided initially a highly sensitive method to measure telomerase activity owing to signal amplification by PCR and improved detergent lysis to allow more uniform extraction of telomerase from a small number of cells (1,11,12).
perform the Telomeric Repeat Amplification Protocol (TRAP) and ELISA assay for non-quantitative detection of telomerase activity in cells and tissues.
To date, telomerase activity has been detected in over 85% of all tumors tested spanning more than 20 different types of cancers (30-31). The TRAPeze® RT Telomerase Detection Kit is a highly sensitive in vitro assay for the fluorometric detection and real time quantification of telomerase activity. It incorporates refinements to the original
The telomeric repeat amplification protocol (TRAP) assay, first published by Kim et al. in 1994 (1), is used to detect telomerase activity. The scope of the technique is far reaching and has implications for telomere, telomerase, aging, and cancer research (2,3).
