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Telomeric repeat amplification protocol (TRAP) is a fast and sensitive PCR-based assay for detection and measurement of telomerase activity. Since its introduction, the TRAP assay has been widely used in cancer and aging studies.
The TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.
The TRAPEZE® XL Kit is a highly sensitive in vitro assay for the fluorometric detection of telomerase activity. It incorporates refinements to the original TRAP assay that were first introduced in the gel-based TRAPEZE® Telomerase Detection Kit (Cat #S7700).
Commercially available kits based on the TRAP assay for detection of telomerase activity have now become accessible. The TRAPeze™ Telomerase Detection Kit is distributed by Oncor (Gaithersburg, MD) and provides lysis buffer and most PCR reaction components of the standard TRAP assay (1).
Telomeric repeat amplification protocol (TRAP) is a fast and sensitive PCR-based assay for detection and measurement of telomerase activity. Since its introduction, the TRAP assay has been widely used in cancer and aging studies.
The standard TRAP utilizes a 150-bp ITAS (internal telomerase assay standard) while the TRAP-eze kit utilizes a short standard of 36 bp [55,60,62]. Most inhibitors affect PCR amplification as they inhibit Taq polymerase activity.
