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The TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.
The telomeric repeat amplification protocol (TRAP) is a two-step process for analyzing telomerase activity in cell or tissue extracts. Recent modifications of this sensitive assay include elimination of radioactivity by using a fluorescently labeled primer instead of a radiolabeled primer.
The TRAPEZE® XL Kit is a highly sensitive in vitro assay for the fluorometric detection of telomerase activity. It incorporates refinements to the original TRAP assay that were first introduced in the gel-based TRAPEZE® Telomerase Detection Kit (Cat #S7700).
The TRAPeze® RT Telomerase Detection Kit is a highly sensitive in vitro assay for the fluorometric detection and real time quantification of telomerase activity. It incorporates refinements to the original TRAP assay that were first introduced in the gel-based TRAPeze® Telomerase Detection Kit (Cat.
The TRAPEZE® XL Kit is a one buffer, two enzyme system utilizing polymerase chain reaction (PCR) and Amplifluor® primers (34). In the first step of the reaction (Figure 1, line A), the telomerase enzyme adds a number of telomeric repeats (GGTTAG) onto the 3' end of a substrate oligonucleotide (TS).
20 lis 2015 · The TRAP assay is a popular method to determine telomerase activity in mammalian cells and tissue samples (Kim et al., 1994). The TRAP assay includes three steps: extension, amplification, and detection of telomerase products.
Commercially available kits based on the TRAP assay for detection of telomerase activity have now become accessible. The TRAPeze™ Telomerase Detection Kit is distributed by Oncor (Gaithersburg, MD) and provides lysis buffer and most PCR reaction components of the standard TRAP assay ( 1 ).
