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The TRAPEZE® Gel-Based Telomerase Detection Kit provides substantial improvements to the original TRAP assay, such as a modified reverse primer sequence which (1) eliminates the need for a wax barrier hot start, (2) reduces amplification artifacts and (3) permits better estimation of telomerase processivity.
The TRAPEZE® XL Kit is a one buffer, two enzyme system utilizing polymerase chain reaction (PCR) and Amplifluor® primers (34). In the first step of the reaction (Figure 1, line A), the telomerase enzyme adds a number of telomeric repeats (GGTTAG) onto the 3' end of a substrate oligonucleotide (TS).
1 wrz 1996 · A PCR-based assay for the detection of telomerase activity (TRAP assay: telomeric repeat amplification protocol) was developed, allowing fast and efficient detection of telomerase activity...
The TRAPeze® RT Telomerase Detection Kit is a highly sensitive in vitro assay for the fluorometric detection and real time quantification of telomerase activity. It incorporates refinements to the original TRAP assay that were first introduced in the gel-based TRAPeze® Telomerase Detection Kit (Cat.
Telomerase activity was measured using the Telo TAGGG Telomerase PCR ELISA diagnostic kit as well as ROCHE - photometric enzyme immunoassay for quantitative determination of telomerase activity,...
Kit, the methodology utilized in TRAP. EZE ® ELISA Kit is based on an improved version of the original method described by Kim et al. (12). The TRAP. EZE ® ELISA Kit is a highly sensitive in vitro assay system for detecting telomerase activity. In addition to those which are necessary for the TRAP assay, the kit includes reagents for ...
1 gru 2010 · Objective —To evaluate the usefulness of a PCR-based telomeric repeat amplification protocol (TRAP) assay for detecting telomerase activity in cells from a canine transitional cell carcinoma (TCC) cell line and, ultimately, in the urine of dogs with TCC.
