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The TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.
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The TRAPeze® RT Telomerase Detection Kit is a highly sensitive in vitro assay for the fluorometric detection and real time quantification of telomerase activity. It incorporates refinements to the original TRAP assay that were first introduced in the gel-based TRAPeze® Telomerase Detection Kit (Cat.
increments starting at 50 nucleotides: 50, 56, 62, 68, etc. The TRAP. EZE ® ELISA Kit (and the gel-based TRAP. EZE ® Kit) utilizes a reverse primer (RP) with modified sequence, instead of the CX primer described originally (12). The modified primer RP eliminates the need for a wax barrier hot start and reduces amplification artifacts.
The TRAPEZE® Gel-Based Telomerase Detection Kit provides substantial improvements to the original TRAP assay, such as a modified reverse primer sequence which (1) eliminates the need for a wax barrier hot start, (2) reduces amplification artifacts and (3) permits better estimation of telomerase processivity.
Telomeric repeat amplification protocol (TRAP) is a fast and sensitive PCR-based assay for detection and measurement of telomerase activity. Since its introduction, the TRAP assay has been widely used in cancer and aging studies.
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1 lip 1997 · The telomeric repeat amplification protocol (TRAP) assay has been used to test telomerase activity in numerous cancer specimens. We describe primers, controls and quantification methods for the TRAP assay to accurately measure the level of telomerase activity in clinical samples.