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OligoEvaluator™ is easy to use: select DNA or RNA, paste your sequence, and click calculate for the OligoEvaluator™ tool to return values. All reported properties are available for export to a convenient Excel template.
- Custom Oligos & qPCR Probes Online Tools
We are pleased to offer OligoEvaluator, our online...
- PCR Assay Optimization and Validation
Optimizing Primer Concentrations and Annealing Temperature...
- Oligonucleotide Melting Temperature
How do you calculate the T m of DNA?. The melting...
- Primer Concentration Optimization Protocol
Steps 1 and 2 are repeated through 40 cycles and followed by...
- Custom & Predesigned Rna Oligos, siRNA Duplexes and Controls
Custom & Predesigned RNA Oligos and siRNA Duplexes MISSION ®...
- Custom & Predesigned DNA Oligos and qPCR Probes
We offer a family of predesigned primers and probe assays...
- Quantitative PCR Basics
A common application of qPCR is gene expression analysis,...
- Quantitative RT-PCR
KiCqStart ® – Instrument Optimized. Our KiCqStart ®...
- Custom Oligos & qPCR Probes Online Tools
Evaluation of methods for the prediction of membrane spanning regions. `Our results show that TMHMM is currently the best performing transmembrane prediction program.' A. Krogh, B. Larsson, G. von Heijne, and E. L. L. Sonnhammer. Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes.
14 cze 2011 · NetPrimer combines the latest primer design algorithms with a web-based interface allowing the user to analyze primers over the Internet. All primers are analyzed for melting temperature using the nearest neighbor thermodynamic theory to ensure accurate Tm prediction.
This tool predicts the melting temperature (Tm) of oligonucleotides enhanced with locked nucleic acids (LNA)
27 mar 2023 · The SignalP and TMHMM plugin contains tools for finding secretory signal peptides and predicting transmembrane helices in protein sequences. This plugin contains two tools: Signal Peptide Prediction and Transmembrane Helix Prediction.
Select ANALYZE for easy, one-click access to a T m calculator, GC content calculator, extinction coefficient calculator, and more. Predict oligo secondary structure or possible duplexes with HAIRPIN, SELF-DIMER, and HETERO-DIMER options. Search for matches to your input oligo sequence in the NCBI BLAST database.
31 sie 2021 · GenAlEx (Genetic Analysis in Excel) is a user-friendly cross-platform package for population genetic analysis that runs within Microsoft Excel(TM). GenAlEx enables population genetic data analysis of codominant, haploid and binary genetic data providing analysis tools applicable to plants, animals and microorganisms.