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10 wrz 2021 · The principle of RNAsnap™ is based on bacteria pyrolysis and formamide-based loading solution for RNA separation from protein and the majority of DNA. This simple protocol holds several advantages which other methods cannot reach, including high efficiency of RNA recovery, fast, and low cost.
4 sty 2022 · Here, we describe recent advances that address these limitations and point to a significant maturation of synthetic RNA-based devices. Synthetic RNA devices integrate sensing,...
1 gru 2016 · Here, we describe a simplified, semi-unified, effective, and toxic material free protocol for extracting DNA and RNA from different prokaryotic and eukaryotic sources exploiting the physical and chemical properties of nucleic acids.
29 sty 2021 · The authors compared DNA and RNA yield, number of quality sequences, microbial community alpha- and beta-diversity and taxonomic composition, limit of detection (LOD) and extent of well-to-well contamination across common sample types and among three extraction protocols.
8 sty 2021 · Here, we describe a universal protocol for simultaneous DNA extraction and sequencing of numerous different bacterial species, allowing mixed species sequence runs to meet variable...
8 gru 2015 · Based on the parameters assessed in this study, Amresco’s Phenol-free total RNA purification kit coupled with an in-house DNase I treatment yielded the best quality RNA extracts comprising low and high molecular weight transcripts with minimal DNA contamination from P. aeruginosa. Bioinformatic analysis of the transcriptome data obtained via ...
29 maj 2024 · To extract a sufficient yield of high quality, purified DNA for your downstream applications, you have to find the best extraction method, or combination of methods, for your sample type. This chapter gives an overview of the most common techniques, explains how they work, and discusses their advantages and disadvantages.
