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Solutions to Practice Problems for Molecular Biology, Session 3: Transcription, Translation!! Question 1 !! Fill in the table: ! Transcription Translation Where does this process occur in a nucleus cytoplasm eukaryotic cell? What is the enzyme that carries out RNA polymerase Ribosome this process? What is the template that is read DNA RNA
NOTE: For this problem, transcription begins with and includes the red and underlined C/G (top strand/bottom strand) base pair and RNA polymerase proceeds from left to right along the DNA. a) Which strand is used as a template for transcription, the top or the bottom?
A scientist observes that a eukaryotic cell has an RNA polymerase deficiency. Describe three additional observations that would together support the conclusion that a defect in RNA polymerase I activity, and not problems with the other polymerases, causes the defect.
RNA polymerase: binds to a DNA molecule and transcribes DNA into mRNA. c. Transcription Factors: respond to external stimuli and bind to specific regions of DNA near the promoter sequence of a gene, which signals RNA transcription to begin. 16. Transcription factors are proteins that bind to promoter regions of DNA and signal RNA polymerase to ...
In this chapter, we have given you problems that will allow you to explore the structure and function of DNA and RNA and how proteins are elaborated in the cell. The first problems examine some of the most important experiments that led to the conclusion that DNA is the genetic material.
RNA polymerase The main enzyme involved in transcription is RNA polymerase, which uses a single-stranded DNA template to synthesize a complementary strand of RNA. Specifically, RNA polymerase builds an RNA strand in the 5' to 3' direction, adding each new nucleotide to the 3' end of the strand. ANA c
Which of the following activities is the mutant polymerase likely to be missing, as compared to the normal polymerase? Circle all that apply. Shown below is a schematic of replicating DNA in a bacterial cell. Region 1. a) On the diagram, label the 5’ and the 3’ ends of the parental DNA strands.
