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4 sty 2020 · Precipitation reactions involve the reaction of soluble antigen with IgG or IgM antibodies to form large interlocking molecular aggregates called lattices.
Precipitation reaction can occurs using polyclonal abs or mixture of monoclonal antibodies. If the antigen is monovalent or a single monoclonal ab is used, no lattice will form. Precipitation reactions may require hours or days to become visible, depending on the type of precipitation reaction.
The precipitation reaction occurs when certain soluble antigens are in contact with the homologus antibodies . In titration of precipitating antigen, three general zones of reaction may be observed, three
Measurement of Precipitation by Light 26 Antigen-antibody complexes, when formed, will precipitate in a solution resulting in a turbid or cloudy appearance that can be measured by: Turbidimetry Nephelometry Passing light through a cloudy solution. (Net decrease in light intensity) Measuring light scattered at a particular
Precipitation reactions occur when a soluble antigen and antibody interact to form an insoluble precipitate. There are two stages: 1) Small antigen-antibody complexes form rapidly. 2) These complexes slowly form lattices that precipitate out of solution.
• Also based on the antigen-antibody reaction. (both can be detected) • Which methods does it include? –Methods based on precipitation –Methods based on agglutination –Immunoassays (ELISA, ELISPOT, radioimmunoassay, etc., see in next practice) –Immunoblot techniques (Western blot, Dot blot, see in next practice)
Precipitation and Agglutination Reactions Most of the serological testing requires the interaction between antibodies and antigens to form visible antibody–antigen complexes. Several methods in clinical serology can detect antigen–antibody reactions, including: 1. Precipitation. 2. Agglutination. 3. Hemagglutination and hemagglutination ...
