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Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.
Understand the expected properties of your oligos before you order them. Calculator for GC content, melting temperature (T m), molecular weight, extinction coefficient, µg/OD, nmol/OD, and more. Identify secondary structure potential. Minimize dimerization. Use NCBI BLAST™.
Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.
21 wrz 2012 · Free tools for oligo design, handling, and storage to help you get the most out of your oligos. Calculate T m, identify secondary structure, optimize codon use, calculate resuspension volume, and more.
The calculator calculates recommended T m (melting temperature) of primers and PCR annealing temperature based on the primer pair sequence, primer concentration, and DNA polymerase used in PCR. The calculator also calculates the primer length, percentage of GC content, molecular weight, and extinction coefficient.
8 lis 2021 · Our Tm calculator accounts for specific buffer compositions that impact primer melting temps. The goal is to avoid weak thermodynamics that can cause primer dimers to form due to inefficient binding.
3 paź 2024 · The IDT Primer Calculator is a helpful tool for researchers and scientists involved in molecular biology. It calculates the melting temperature (Tm) of a DNA primer, which is essential for designing efficient polymerase chain reaction (PCR) experiments.
