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Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.
Research-friendly oligo calculator. Flexible input and advanced parameters to optimize your custom order: Enter your primer or other oligo sequence; Adjust calculation options if desired; Choose a function: Select ANALYZE for easy, one-click access to a T m calculator, GC content calculator, extinction coefficient calculator, and more.
The calculator calculates recommended T m (melting temperature) of primers and PCR annealing temperature based on the primer pair sequence, primer concentration, and DNA polymerase used in PCR. The calculator also calculates the primer length, percentage of GC content, molecular weight, and extinction coefficient.
Tm Calculator Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.
Simplify planning of your qPCR experiments using IDT free, online tools for oligonucleotide analysis and PCR primer design. This article provides an overview of our predesigned qPCR sequences and the basics of designing customized PCR primers and hydrolysis probes with the PrimerQuest™ Tool.
21 paź 2013 · The free, online IDT OligoAnalyzer Tool allows researchers to calculate T m for mismatched sequences. You can also use the program to determine probe locations that help enable better mismatch discrimination.
Browse NEB's beta tools, including REBSITES, Primer Monitor, Tm API, Glycan Analyzer Code and Genomes and Restriction Enzyme Frequencies, NEBNext Index Oligo Selector.
