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Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.
OligoAnalyzer™ Tool. Understand the expected properties of your oligos before you order them. Calculator for GC content, melting temperature (T m), molecular weight, extinction coefficient, µg/OD, nmol/OD, and more. Identify secondary structure potential. Minimize dimerization.
Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.
The calculator calculates recommended T m (melting temperature) of primers and PCR annealing temperature based on the primer pair sequence, primer concentration, and DNA polymerase used in PCR. The calculator also calculates the primer length, percentage of GC content, molecular weight, and extinction coefficient.
15 cze 2012 · OligoAnalyzer calculates Tm, molecular weight, hairpins, self-dimers, heterodimers, BLAST search, and Tm mismatch for custom DNA or RNA oligos.
24 sie 2015 · Simplify planning of your qPCR experiments using IDT free, online tools for oligonucleotide analysis and PCR primer design. This article provides an overview of our predesigned qPCR sequences and the basics of designing customized PCR primers and hydrolysis probes with the PrimerQuest™ Tool.
Learn how to use the IDT Primer Calculator to find the melting temperature of your DNA sequence. Understand the formula behind it, get a step-by-step example, and find answers to frequently asked questions. Plus, create your own HTML code for an interactive calculator.
