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11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
1 cze 2023 · NOD mouse dorsal root ganglia display morphological and gene expression defects before and during autoimmune diabetes development.
Though H&E stains are relatively simple to perform, there are a variety of problems that can occur. Learn how to troubleshoot common H&E staining issues.
The H&E stain provides a comprehensive picture of the microanatomy of organs and tissues. Hematoxylin precisely stains nuclear components, including heterochromatin and nucleoli, while eosin stains cytoplasmic components including collagen and elastic fibers , muscle fibers and red blood cells.
Always review sections using the basic hematoxylin and eosin (H&E) stain before proceeding to perform an immunohistochemical assay in order to check out the morphology of the tissue and to determine
23 lut 2024 · The histopathological examination of tissue is the cornerstone of cancer diagnosis globally. It is based on the staining of tissue samples with histochemical dyes, such as haematoxylin and eosin (H&E), to highlight cellular components for visual interpretation by pathologists.
This protocol describes manual H&E staining of fixed, processed, paraffin-embedded, and sectioned mouse tissues. In H&E-stained tissues, the nucleic acids stain dark blue and the proteins stain red to pink or orange.
