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11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
Always review sections using the basic hematoxylin and eosin (H&E) stain before proceeding to perform an immunohistochemical assay in order to check out the morphology of the tissue and to determine
24 gru 2013 · A Practical Guide to the Histology of the Mouse provides a full–colour atlas of mouse histology, aimed at veterinary and medical pathologists who are unfamiliar with mouse tissues and scientists who wish to evaluate their own mouse models.
Important points to remember for mouse brain histology : --Perfusion fixation is important to avoid artefacts, such as “dark neurons” --Do not leave in 70% alcohol for longer than 24 hours, to avoid vacuole artefact --both erythrocytes and degenerating neurons are autofluorescent. --To avoid hypostatic congestion in multiple tissues and to
21 cze 2024 · Sensory neurons in the dorsal root ganglion (DRG) and trigeminal ganglion (TG) are specialized to detect and transduce diverse environmental stimuli to the central nervous system.
H&E-stained sections of dorsal root ganglia (DRG) were evaluated blindly for histopathological lesions by a board-certified pathologist (A.D.M.); scoring was based on the presence and...
27 gru 2013 · A systematic approach to the mouse necropsy, which allows the examination of all the tissues in the animal in the most expedient manner, is recommended. This chapter describes a recommended ...
