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11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
Hematoxylin and Eosin stains are used in many areas of the histology laboratory, including frozen sections, fine needle aspirates, and paraffin fixed embedded tissues. To better understand what makes a well-stained slide, it is important to understand the components of the stain.
23 lut 2024 · In this paper we propose a method for absolute quantification of H&E staining in the laboratory environment, using stain assessment slides. Stain assessment slides comprise of a biopolymer film applied as a label to standard pathology glass slides.
This protocol describes manual H&E staining of fixed, processed, paraffin-embedded, and sectioned mouse tissues. In H&E-stained tissues, the nucleic acids stain dark blue and the proteins stain red to pink or orange.
The laboratory provides comprehensive histology services for a wide variety of organs and tissues derived from different species of experimental animals (e.g., mouse, rat, pig, sheep, zebrafish) for routine histology, immunostainings and nucleic acid hybridization analysis.
27 gru 2013 · A Practical Guide to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year.
Hematoxylin and Eosin Staining. For routine examination, haematoxylin and eosin (H&E) is the stain of choice. H&E produces colors different tissue structures, which would otherwise be transparent, to get detailed view of the tissue.
