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11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
23 sty 2023 · Our integrative and comparative analysis of DRGs from mice, guinea pigs, cynomolgus monkeys, and humans identified diverse subtypes of DRG sensory neurons in each species and showed that these...
1 cze 2023 · Histology. Some ganglia were harvested and fixed with 4% PFA and then included in paraffin blocks. 12 µm sections were stained with routine hematoxylin and eosin (H&E) protocol. Electronic microscopy. Some ganglia were harvested and fixed with 4% PFA and then fixed with 2.5% glutaraldehyde in 0.1M phosphate buffer (pH 7.4) for 24 hours.
12 maj 2023 · Virtual staining of unstained histological tissue by using deep neural networks has the potential to streamline the sample processing phase in histopathology and to reduce material consumption.
4 mar 2024 · We used this resource to reveal a remarkable degree of morphological and physiological diversity across the transcriptionally distinct DRG neuron subtypes. This mouse DRG neuron toolkit should enable analyses of the neuronal building blocks of somatosensation.
H&E-stained sections of dorsal root ganglia (DRG) were evaluated blindly for histopathological lesions by a board-certified pathologist (A.D.M.); scoring was based on the presence and...
13 kwi 2022 · Here, we quantified the innervation of the mouse lung by vagal and dorsal root ganglion (DRG) sensory subsets defined by the expression of Pirt (all afferents), 5HT 3 (vagal nodose afferents), Tac1 (tachykinergic afferents), and transient receptor potential vanilloid 1 channel (TRPV1; defensive/nociceptive afferents) using Cre-mediated reporter ...
