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Remel MacConkey Broth is a liquid medium recommended for use in qualitative procedures for selective and differential isolation of enteric gram-negative bacilli. SUMMARY AND EXPLANATION.
BACGroTM MacConkey Broth, when prepared as directed, is used as a selective media for the growth of coliform bacteria. It is not intended for use in diagnosis, treatment, or prevention of disease in humans. BACGroTM MacConkey Broth conforms to harmonized USP/EP/JP requirements.
charts to find MPN (Most Probabile Number) of total 100 ml sample. For the differential test, subculture from each tube showing positive reaction into a fresh tube of MacConkey Broth.
Technical Specification Sheet. MacConkey Broth (NCM0060) Intended Use . MacConkey Broth is used for the detection of coliform bacteria in milk and water. Conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans. Description.
Directions. Suspend 40.01 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense into test tubes with inverted Durham tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubes to 45-50°C before inoculation. Principle And Interpretation.
MacConkey Broth is a liquid medium used for the selective cultivation of Gram-negative, lactose-fermenting bacilli in water, food and pharmaceutical raw materials as a presumptive test for coliform organisms.
Preparation Instructions. Suspend 40.00 grams of MacConkey Broth in 1000 mls of distilled water. Heat to dissolve the medium completely. Distribute into tubes containing inverted Durham’s tubes and sterilize by autoclaving at 15. ProductInformation. lbs. pressure (121 C) for 15 minutes. Cool the tubes before inoculation. Storage.
