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  1. The lasers commonly employed in optical microscopy are high-intensity monochromatic light sources, which are useful as tools for a variety of techniques including optical trapping, lifetime imaging studies, photobleaching recovery, and total internal reflection fluorescence.

    • Confocal Microscopy

      Laser scanning confocal microscopy (LSCM) is a tool that has...

    • Blog

      How to Clean and Sterilize Your Microscope. Featured Post....

  2. Diode lasers are available in a range of wavelengths spanning from the ultraviolet to the near infrared, with output power substantial enough for confocal microscopy applications. In addition, those diode lasers exhibit improved beam shape and stability, which has enabled them to replace gas lasers in practically all applications.

  3. In reality, the output mirror always transmits a constant fraction of the light as the beam, reflecting the rest back into the cavity. This function is important in allowing the laser to reach an equilibrium state, with the power levels both inside and outside the laser becoming constant.

  4. The primary functions of a confocal microscope are to produce a point source of light and reject out-of-focus light, which provides the ability to image deep into tissues with high resolution, and optical sectioning for 3D reconstructions of imaged samples.

  5. en.wikipedia.org › wiki › Laser_diodeLaser diode - Wikipedia

    A laser diode (LD, also injection laser diode or ILD or semiconductor laser or diode laser) is a semiconductor device similar to a light-emitting diode in which a diode pumped directly with electrical current can create lasing conditions at the diode's junction.

  6. 30 kwi 2022 · Laser scanning microscopy is used for precision measurements, while time-resolved (sub-nano) detection methods in combination with picosecond laser excitation are used to distinguish different species, e.g., monomers, aggregates or ionic species, on the basis of their fluorescence lifetimes.

  7. 24 lut 2020 · A laser diode with an emission wavelength of λ = 405 nm (continuous-wave output power 5 mW) was used to excite the QDs in the sample, resulting in light emission in the red spectral range...

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