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Though H&E stains are relatively simple to perform, there are a variety of problems that can occur. Learn how to troubleshoot common H&E staining issues.
H&E Troubleshooting Table . Page 1 of 7 . Problem Cause Solution . 1. Nuclei not crisp, “smudgy” nuclei, nuclear bubbling or no distinct chromatin pattern seen. There is no variation in the nuclear chromatin patterns among cells, and there is no variation in chromatin staining within one nucleus. a) Fixation is incomplete.
The H&E procedure stains the nucleus and cytoplasm contrasting colors to readily differentiate cellular components. Though the H&E staining is a relatively simple method to perform, there are a variety of artifacts that can interfere with a good stain.
Review the basics of H&E staining and what optimal staining should look like. Identify common problems encountered with staining techniques. Consider solutions to common artifacts that influence stain results.
28 sie 2018 · TROUBLESHOOTING IN H&E STAIN Pale staining with eosin. The pH of the eosin solution may be above 5.0, possibly caused by carryover of the bluing reagent. Check the pH of the eosin solution, and adjust it to a pH of 4 to 5 with acetic acid if necessary.
9 gru 2014 · The complete method for H&E staining is contained in the tables below, but we’ll take a look at each of the stages in turn and explain the process which should help you should you need to troubleshoot.
15 maj 2020 · In this post we will cover some common problems you may run into. The slide is too pink. If the slide is too pink it is generally a result of your eosin and can be solved by altering the time that you leave the slide in various steps within the H&E staining process.
