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1. en.wikipedia.org › wiki › DNA_polymerase_III_holoenzymeDNA polymerase III holoenzyme - Wikipedia

   en.wikipedia.org › wiki › DNA_polymerase_III_holoenzyme

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   DNA polymerase III holoenzyme is the primary enzyme complex involved in prokaryotic DNA replication. It was discovered by Thomas Kornberg (son of Arthur Kornberg) and Malcolm Gefter in 1970.

2. www.jbc.org › article › S0021-9258(19)77120-8Assembly of DNA Polymerase III Holoenzyme

   www.jbc.org › article › S0021-9258(19)77120-8

   We developed a new assay based on the binding of complexes containing biotin-tagged τ to streptavidin-coated agarose beads to investigate the effects of various DNA polymerase III holoenzyme subunits on the kinetics of co-assembly of γ and τ into the same complex.

3. www.cell.com › fulltext › S0092-8674(01)00400-7The DNA Polymerase III Holoenzyme - Cell Press

   www.cell.com › fulltext › S0092-8674(01)00400-7

   The DNA polymerase III holoenzyme (holoenzyme) contains two pol III cores (Pol III) that are held together by a τ dimer (McHenry, 1982; Studwell-Vaughan and O'Donnell, 1991).

4. www.ncbi.nlm.nih.gov › pmc › articlesLong-Range PCR Amplification of DNA by DNA Polymerase III...

   www.ncbi.nlm.nih.gov › pmc › articles

   19 sty 2015 · DNA replication in bacteria is accomplished by a multicomponent replicase, the DNA polymerase III holoenzyme (pol III HE). The three essential components of the pol III HE are the α polymerase, the β sliding clamp processivity factor, and the DnaX clamp-loader complex.

5. www.jbc.org › article › S0021-9258(18)80346-5Fidelity of Escherichia coli DNA Polymerase III Holoenzyme

   www.jbc.org › article › S0021-9258(18)80346-5

   We measured the lifetime of pol III holoenzyme on p/t DNA using three different templates: a 100-mer, a 209-nt restriction fragment of M13 DNA, and full-length circular M13 DNA. Pol III holoenzyme was loaded onto a labeled p/t DNA in the presence of dGTP and dTTP allowing the polymerase to idle.

6. www.jbc.org › article › S0021-9258(19)84209-6Role of the Core DNA Polymerase III Subunits at the Replication...

   www.jbc.org › article › S0021-9258(19)84209-6

   The DNA polymerase III holoenzyme is composed of 10 subunits. The core of the polymerase contains the catalytic polymerase subunit, α, the proofreading 3′ → 5′ exonuclease, ε, and a subunit of unknown function, θ.

7. www.cell.com › fulltext › S0092-8674(00)80069-0DNA Polymerase III: Running Rings around the Fork - Cell Press

   www.cell.com › fulltext › S0092-8674(00)80069-0

   12 sty 1996 · Addition of the β subunit to pol III* regenerates the holoenzyme and restores processivity. The pol III* complex can be reconstituted from individual subunits, and a general picture of its overall organization has been deduced from detailed analysis of subunit–subunit interactions (Onrust et al. 1995references therein) .