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Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy.
Chlorophyll is best extracted from microalgae by supercritical fluid extraction method. High performance liquid chromatography was found to be the most accurate and sensitive technique to fractionate and to quantify chlorophyll along with its derivatives.
8 lut 2020 · Reversed-phase supercritical fluid chromatography (RP-SFC), which can be achieved with a non-polar C18-bonded phase, is an interesting alternative for the separation of this type of non-polar compounds. With regards to the organic solvents used in NARP-LC, carbon dioxide has many advantages: ecological, economic and physico-chemical.
9 sie 2020 · A deeper analysis has been achieved through the mass spectrophotometric (MS) studies of chlorophyll compounds of main microalgae and cyanobacteria, including the analysis of the fragmentation pathways to increase consistency of the identification.
18 sie 2018 · High performance liquid chromatography (HPLC) is one of the best options to characterise the exact pigment composition in a plant tissue, and it is the standard method to quantify individual carotenoids.
Carotenoids are robust molecules, relatively stable during the extraction procedure. Carotenoids are lipophilic molecules, and a non-polar solvent is required for their extraction. A certain amount of water (1-5%) favours the extraction of some xanthophylls.
Chlorophyll a is slightly soluble in a 3:1:1 mixture of petroleum ether, acetone, and water. Carotenoids are very soluble in this solvent system. These solubility differences will allow the separation of chlorophyll a from the carotenoids and chlorophyll b on a paper chromatogram.
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powiązane z: best solvent for chlorophyll chromatography and mass waterOur solvents and additives are designed to have the lowest levels of impurities. Maintain the highest standards of performance with high-quality parts.