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1. www.sciencedirect.com › science › articleDevelopment of an analytical method for chlorophyll pigments...

   www.sciencedirect.com › science › article

   8 lut 2020 · An improved high performance liquid chromatography-photodiode array detection-atmospheric pressure chemical ionization-mass spectrometry method for determination of chlorophylls and their derivatives in freeze-dried and hot-air-dried Rhiacanthus nasutus (L.) Kurz

2. link.springer.com › chapter › 10Analytical Protocols in Chlorophyll Analysis | SpringerLink

   link.springer.com › chapter › 10

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   9 sie 2020 · A deeper analysis has been achieved through the mass spectrophotometric (MS) studies of chlorophyll compounds of main microalgae and cyanobacteria, including the analysis of the fragmentation pathways to increase consistency of the identification.

3. www.scienceinschool.org › article › 2019Colour, chlorophyll and chromatography – Science in School

   www.scienceinschool.org › article › 2019

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   12 cze 2019 · Why is it important to use a nonpolar solvent (such as hexane, acetone and trichloromethane) and not a polar solvent (such as water) to investigate plant pigments using thin-layer chromatography? Why should you avoid touching the thin-layer chromatography plate?

4. prometheusprotocols.net › determination-of-chlorophylls-and-carotenoids-by-hplcDetermination of chlorophylls and carotenoids by HPLC

   prometheusprotocols.net › determination-of-chlorophylls-and-carotenoids-by-hplc

   The solvents must be HPLC grade or vacuum filtered through a nylon membrane of 0.2 μm pore size before use (as for the Tris buffer in solvent A) in order to avoid problems in the accuracy of the system.

5. www.chem.ucla.edu › ~bacher › CHEM14CLIsolation of Chlorophyll and Carotenoid Pigments from Spinach

   www.chem.ucla.edu › ~bacher › CHEM14CL

   The major advantages of column chromatography are its ability to handle larger amounts of material (compared to GC, TLC and HPLC) and the ability to change the eluting solvent throughout the course of the separation. This allows one to remove one component while a desired product remains essentially unmoved.

6. www.purdue.edu › science › science-expressANALYSIS OF PLANT PIGMENTS USING PAPER CHROMATOGRAPHY - Purdue...

   www.purdue.edu › science › science-express

   Chlorophyll a is slightly soluble in a 3:1:1 mixture of petroleum ether, acetone, and water. Carotenoids are very soluble in this solvent system. These solubility differences will allow the separation of chlorophyll a from the carotenoids and chlorophyll b on a paper chromatogram.

7. weissman.baruch.cuny.edu › wp-content › uploadsPaper Chromatography of Pigments in a Spinach Leaf - City...

   weissman.baruch.cuny.edu › wp-content › uploads

   Obtain a chromatography chamber (a beaker covered with a watch glass or aluminum foil) and add developing solvent (a mixture of petroleum ether and acetone). The solvent should completely cover the bottom of the chamber to a depth of approximately 0.5 cm. Obtain chromatography paper.