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Basics of Pipetting Handbook. Your Guide to Getting the Most Out of Your Pipette. Table of Contents. Your pipette is a precision instrument. Master the basics of pipetting to get the best results from your assays through good pipetting techniques and practices.
Rest position. First stop. cond stop (purge)2AspirationImmerse. he pipette tip in the liquid*. Allow the plunger to move up. smoothly to the rest position. Wait one second so that all the liquid ha. ve up into the tip.3DispensePlace the pipette tip at an angle (10° to 45°) against the insid.
Good Laboratory Pipetting practices significantly influence the results of your liquid handling. The performance of your pipette is key and is a result of several factors, which include ensuring desired specifications are met.
pipetting handbook is an indispensable reference tool for new and experienced pipette users alike. Lastly, although examples in this handbook may refer to Rainin pipettes and data, the principles as techniques apply to any brand of pipette.
Errors resulting from poor pipetting techniques can significantly affect test results, as proper pipetting plays an important role in achieving accurate and repeatable measurements. Here are some tips on how to pipet properly: Corning® Lambda® Plus single-channel pipettor. Corning Lambda Plus 8-channel pipettor.
INTRODUCTION TO MICRO-PIPETTING. Katherine Dorfman UMass Biology Department 2019. Goal for this lab: Master micropipetting technique. Introduction. bility to use a micropipettor. Micropipettors are used to make accurate measurements of extremely small volumes—from one milliliter down to.
Pipetting Techniques. Best pipetting practice. 1) Correct pipette/tip combination. Reduce errors by choosing a pipette/tip combination with the smallest possible air cushion. 2) Correct immersion depth. Immerse as little as possible. preventing liquid transfer on the tip’s outside. Immerse deeply enough to avoid uptake of air.
