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In both cases, a tightly regulated expression system is critical for maximizing recombinant protein yields. Based on the araBAD operon, which controls the arabinose metabolic pathway in E. coli (1,2,3), the pBAD Expression System allows you to precisely modulate lev-els to optimize yields.
1 lis 2015 · To find whether the hypothesis was supported or failed to be supported, the experiment analyzed differential expression of GFP from a transformation of MM294 E. coli with pGLO plasmid and used...
The pBAD expression system allows tightly controlled, titratable expression of your protein through the regulation of specific carbon sources such as glucose, glycerol, and arabinose. pBAD is ideal for expressing toxic proteins and optimizing protein solubility in E. coli.
With the pGLO transformation kit, students use a simple procedure to transform bacteria with a gene that codes for Green Fluorescent Protein (GFP). The real-life source of this gene is the bioluminescent jellyfish Aequorea victoria, and GFP causes the jellyfish to fluoresce and glow in the dark. Following the transformation procedure, the bacteria
The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products.
The Fold ′n′ Glow Protein Solubility Assay kit allows a test protein to be expressed as an N-terminal fusion with a fluorescent protein [i.e., Green Fluorescent Protein (GFP), Cyan Fluorescent Protein (CFP), and Yellow Fluorescent Protein (YFP)].
21 lip 2021 · We tested a ΔprgH mutant (type III secretion system protein encoded on SPI-1) and a Δspi1 mutant lacking the entire SPI-1 operon alone, or combined with deletions in araA and araE, for their ability to form biofilms in the presence or absence of L-arabinose.
