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5 cze 2021 · Result Interpretation of Spore Stain (Schaeffer-Fulton Method) Examples. Interesting Science Videos. Objectives of Spore Stain. To prepare an endospore stain of bacterial cells and demonstrate endospores in the stained preparation. To differentiate between vegetative cells and endospores. Principle of Spore Stain.
Learn to perform the spore stain. Identify spores on a bacterial smear. Endospore production is a very important characteristic of some bacteria, allowing them to resist adverse environmental conditions such as desiccation, chemical exposure, extreme heat, etc.
The spore stain involves four steps: primary staining, steaming, decolorizing, and counterstaining. In the primary staining step, the smear is flooded with malachite green and heated over a water bath. The heat helps the dye to penetrate the spore coat and stain the endospores green.
Interpret results of an endospore stain. Identify when endospores are terminal, subterminal, and central in microscopic images, diagrams, and descriptions. Tell how the endospore stain works including the stains involved and how the stains penetrate cells and do or do not wash out of cells.
10 sie 2022 · Endospore Staining- Principle, Reagents, Procedure and Result. The main purpose of endospore staining is to differentiate bacterial spores from other vegetative cells and to differentiate spore formers from non-spore formers.
13 kwi 2016 · This paper reviewed core concepts of interpreting bacterial culture results, including timing of cultures, common culture sites, potential for contamination, interpreting the Gram stain, role of rapid diagnostic tests, conventional antibiotic susceptibility testing, and automated testing.
Interpretation. Spore-forming bacteria are indicated by the presence of green-stained spores in a bacterial culture. The morphology of the spores—their size, form, and inside-cell location—can be utilized to determine the type of bacteria. Important Note
