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  1. Compute pI/Mw is a tool that calculates the theoretical isoelectric point and molecular weight of protein sequences from UniProtKB entries or user input. You can enter one or more UniProtKB AC/ID or a single letter code, and choose the resolution mode (average or monoisotopic).

    • Documentation

      If your protein of interest is not in Swiss-Prot/TrEMBL, you...

    • Figure 2

      Compute pI/Mw Tool - Sample Output. Fig 2: Sample output...

    • ProtParam

      ProtParam [ Documentation / Reference ] is a tool which...

  2. Calculate the molecular weight of a protein sequence and compare it with a set of protein standards. Append commonly used epitopes and fusion proteins to the sequence and paste it into the text area.

  3. 5 cze 2023 · Calculate the molecular weight of a protein in kDa and u by selecting the amino acids that comprise it. Learn how to use the calculator, how to convert amino acids to kDa, and why it is useful for Western Blot technique.

  4. Calculate the mw and extinction coefficient of an oligopeptide or protein based on its amino acid sequence. This tool is useful for determining the concentration of a peptide or protein solution and has been cited in 7 publications.

  5. ProtParam computes molecular weight, theoretical pI, amino acid composition and other physical and chemical parameters for a protein sequence. Enter a UniProtKB AC/ID or a protein sequence in single letter code to use this tool.

  6. Compute the theoretical pI (isoelectric point) and Mw (molecular weight) for a list of UniProt Knowledgebase (Swiss-Prot or TrEMBL) entries or for user entered sequences.

  7. Use this tool to evaluate your protein or peptide sequence length, molecular weight and extinction coefficient. Enter your sequence in the yellow box and hit update to calculate or reset.

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