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26 sty 2024 · We optimized the UV dosage to efficiently cross-link RNA and proteins in plants and expressed epitope-tagged RBPs under the control of their native promoters in loss-of-function mutants.
1 kwi 2024 · (D) CLIP method to map the protein-RNA interactions at nucleotide resolution. In CLIP, plant cells are subjected to UV-C treatment, resulting in the formation of a covalent bond between protein and RNA. The covalent bond creates a signature in reverse transcription and sequencing.
23 lip 2021 · Metrics. Abstract. DNA–protein interactions are essential for several molecular and cellular mechanisms, such as transcription, transcriptional regulation, DNA modifications, among others. For many decades scientists tried to unravel how DNA links to proteins, forming complex and vital interactions.
11 mar 2021 · Abstract. RNAs transmit information from DNA to encode proteins that perform all cellular processes and regulate gene expression in multiple ways. From the time of synthesis to degradation, RNA molecules are associated with proteins called RNA-binding proteins (RBPs).
11 mar 2021 · Antiviral RNA silencing in plants occurs via the production of small RNAs from viral RNAs by dicer-like protein 4 (DCL4), as targeted by the interacting partner of DCL4, double-stranded RNA-binding protein 4 (DRB4), which binds double-stranded RNA of turnip yellow mosaic virus (TYMV) .
27 paź 2021 · In this Review, we discuss evidence from different plants and animals that small RNAs can act in a non-cell-autonomous manner and even exchange information between species.
1 paź 2014 · Protein–RNA structures are shown in blue and protein–DNA structures in green, with protein in the darker shade. π-stacking interactions play a prominent part in both the single-stranded DNA ...
